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human liver epithelial cells l02  (Keygen Biotech)

 
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    Keygen Biotech human liver epithelial cells l02
    Chemokine CXC receptor 4 (CXCR4) is activated by nontypeable Haemophilus influenzae (NTHi) in <t>epithelial</t> cells of middle ear. (A) NTHi was used to treat human middle ear epithelial cells (HMEECs) (25, 50 and 250 for MOI) for 6 h, followed by measurement of CXCR4 gene levels through RT-qPCR analysis. (B) CXCR4 mRNA levels were detected via RT-qPCR analysis in NTHi (MOI of 50)-treated HMEECs cells for different times as indicated. (C) HMEECs were exposed to NTHi for 6 h, and CXCR4 expression from protein levels was evaluated through enzyme-linked immunosorbent assay (ELISA) method. (D) The renal epithelial cells (HK2), (E) liver epithelial <t>L02</t> cells, and (F) human lung epithelial cells (BEAS-2B), as well as (G) mouse lung epithelial cells (MLE-12) were treated with NTHi for different times, and the CXCR4 mRNA levels were tested through RT-qPCR assays. (H) HMEECs were exposed to various strains of NTHi (2019, 12 and 2866) for 6 h. RT-qPCR analysis was applied to explore CXCR4 gene levels. (I) The experimental animals were inoculated with NTHi for various times as indicated, and CXCR4 mRNA levels were calculated in the the middle ear tissue samples through RT-qPCR assays. The representative data are shown as SEM *** p<0.001 vs. the control group without any treatments.
    Human Liver Epithelial Cells L02, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human liver epithelial cells l02/product/Keygen Biotech
    Average 90 stars, based on 1 article reviews
    human liver epithelial cells l02 - by Bioz Stars, 2026-05
    90/100 stars

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    1) Product Images from "Quercetin inhibits NTHi-triggered CXCR4 activation through suppressing IKKα/NF-κB and MAPK signaling pathways in otitis media"

    Article Title: Quercetin inhibits NTHi-triggered CXCR4 activation through suppressing IKKα/NF-κB and MAPK signaling pathways in otitis media

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3577

    Chemokine CXC receptor 4 (CXCR4) is activated by nontypeable Haemophilus influenzae (NTHi) in epithelial cells of middle ear. (A) NTHi was used to treat human middle ear epithelial cells (HMEECs) (25, 50 and 250 for MOI) for 6 h, followed by measurement of CXCR4 gene levels through RT-qPCR analysis. (B) CXCR4 mRNA levels were detected via RT-qPCR analysis in NTHi (MOI of 50)-treated HMEECs cells for different times as indicated. (C) HMEECs were exposed to NTHi for 6 h, and CXCR4 expression from protein levels was evaluated through enzyme-linked immunosorbent assay (ELISA) method. (D) The renal epithelial cells (HK2), (E) liver epithelial L02 cells, and (F) human lung epithelial cells (BEAS-2B), as well as (G) mouse lung epithelial cells (MLE-12) were treated with NTHi for different times, and the CXCR4 mRNA levels were tested through RT-qPCR assays. (H) HMEECs were exposed to various strains of NTHi (2019, 12 and 2866) for 6 h. RT-qPCR analysis was applied to explore CXCR4 gene levels. (I) The experimental animals were inoculated with NTHi for various times as indicated, and CXCR4 mRNA levels were calculated in the the middle ear tissue samples through RT-qPCR assays. The representative data are shown as SEM *** p<0.001 vs. the control group without any treatments.
    Figure Legend Snippet: Chemokine CXC receptor 4 (CXCR4) is activated by nontypeable Haemophilus influenzae (NTHi) in epithelial cells of middle ear. (A) NTHi was used to treat human middle ear epithelial cells (HMEECs) (25, 50 and 250 for MOI) for 6 h, followed by measurement of CXCR4 gene levels through RT-qPCR analysis. (B) CXCR4 mRNA levels were detected via RT-qPCR analysis in NTHi (MOI of 50)-treated HMEECs cells for different times as indicated. (C) HMEECs were exposed to NTHi for 6 h, and CXCR4 expression from protein levels was evaluated through enzyme-linked immunosorbent assay (ELISA) method. (D) The renal epithelial cells (HK2), (E) liver epithelial L02 cells, and (F) human lung epithelial cells (BEAS-2B), as well as (G) mouse lung epithelial cells (MLE-12) were treated with NTHi for different times, and the CXCR4 mRNA levels were tested through RT-qPCR assays. (H) HMEECs were exposed to various strains of NTHi (2019, 12 and 2866) for 6 h. RT-qPCR analysis was applied to explore CXCR4 gene levels. (I) The experimental animals were inoculated with NTHi for various times as indicated, and CXCR4 mRNA levels were calculated in the the middle ear tissue samples through RT-qPCR assays. The representative data are shown as SEM *** p<0.001 vs. the control group without any treatments.

    Techniques Used: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Control



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    human liver epithelial cells l02  (Keygen Biotech)
    90
    Keygen Biotech human liver epithelial cells l02
    Chemokine CXC receptor 4 (CXCR4) is activated by nontypeable Haemophilus influenzae (NTHi) in <t>epithelial</t> cells of middle ear. (A) NTHi was used to treat human middle ear epithelial cells (HMEECs) (25, 50 and 250 for MOI) for 6 h, followed by measurement of CXCR4 gene levels through RT-qPCR analysis. (B) CXCR4 mRNA levels were detected via RT-qPCR analysis in NTHi (MOI of 50)-treated HMEECs cells for different times as indicated. (C) HMEECs were exposed to NTHi for 6 h, and CXCR4 expression from protein levels was evaluated through enzyme-linked immunosorbent assay (ELISA) method. (D) The renal epithelial cells (HK2), (E) liver epithelial <t>L02</t> cells, and (F) human lung epithelial cells (BEAS-2B), as well as (G) mouse lung epithelial cells (MLE-12) were treated with NTHi for different times, and the CXCR4 mRNA levels were tested through RT-qPCR assays. (H) HMEECs were exposed to various strains of NTHi (2019, 12 and 2866) for 6 h. RT-qPCR analysis was applied to explore CXCR4 gene levels. (I) The experimental animals were inoculated with NTHi for various times as indicated, and CXCR4 mRNA levels were calculated in the the middle ear tissue samples through RT-qPCR assays. The representative data are shown as SEM *** p<0.001 vs. the control group without any treatments.
    Human Liver Epithelial Cells L02, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human liver epithelial cells l02/product/Keygen Biotech
    Average 90 stars, based on 1 article reviews
    human liver epithelial cells l02 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    Chemokine CXC receptor 4 (CXCR4) is activated by nontypeable Haemophilus influenzae (NTHi) in epithelial cells of middle ear. (A) NTHi was used to treat human middle ear epithelial cells (HMEECs) (25, 50 and 250 for MOI) for 6 h, followed by measurement of CXCR4 gene levels through RT-qPCR analysis. (B) CXCR4 mRNA levels were detected via RT-qPCR analysis in NTHi (MOI of 50)-treated HMEECs cells for different times as indicated. (C) HMEECs were exposed to NTHi for 6 h, and CXCR4 expression from protein levels was evaluated through enzyme-linked immunosorbent assay (ELISA) method. (D) The renal epithelial cells (HK2), (E) liver epithelial L02 cells, and (F) human lung epithelial cells (BEAS-2B), as well as (G) mouse lung epithelial cells (MLE-12) were treated with NTHi for different times, and the CXCR4 mRNA levels were tested through RT-qPCR assays. (H) HMEECs were exposed to various strains of NTHi (2019, 12 and 2866) for 6 h. RT-qPCR analysis was applied to explore CXCR4 gene levels. (I) The experimental animals were inoculated with NTHi for various times as indicated, and CXCR4 mRNA levels were calculated in the the middle ear tissue samples through RT-qPCR assays. The representative data are shown as SEM *** p<0.001 vs. the control group without any treatments.

    Journal: International Journal of Molecular Medicine

    Article Title: Quercetin inhibits NTHi-triggered CXCR4 activation through suppressing IKKα/NF-κB and MAPK signaling pathways in otitis media

    doi: 10.3892/ijmm.2018.3577

    Figure Lengend Snippet: Chemokine CXC receptor 4 (CXCR4) is activated by nontypeable Haemophilus influenzae (NTHi) in epithelial cells of middle ear. (A) NTHi was used to treat human middle ear epithelial cells (HMEECs) (25, 50 and 250 for MOI) for 6 h, followed by measurement of CXCR4 gene levels through RT-qPCR analysis. (B) CXCR4 mRNA levels were detected via RT-qPCR analysis in NTHi (MOI of 50)-treated HMEECs cells for different times as indicated. (C) HMEECs were exposed to NTHi for 6 h, and CXCR4 expression from protein levels was evaluated through enzyme-linked immunosorbent assay (ELISA) method. (D) The renal epithelial cells (HK2), (E) liver epithelial L02 cells, and (F) human lung epithelial cells (BEAS-2B), as well as (G) mouse lung epithelial cells (MLE-12) were treated with NTHi for different times, and the CXCR4 mRNA levels were tested through RT-qPCR assays. (H) HMEECs were exposed to various strains of NTHi (2019, 12 and 2866) for 6 h. RT-qPCR analysis was applied to explore CXCR4 gene levels. (I) The experimental animals were inoculated with NTHi for various times as indicated, and CXCR4 mRNA levels were calculated in the the middle ear tissue samples through RT-qPCR assays. The representative data are shown as SEM *** p<0.001 vs. the control group without any treatments.

    Article Snippet: The human liver epithelial cells (L02) and human lung epithelial cells (BEAS-2B) were purchased from the KeyGen Biotech (Nanjing, China).

    Techniques: Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Control